Preparation of monoclonal antibodies for the diagnosis of influenza A infection using different immunization protocols
Identifieur interne : 001D05 ( Main/Exploration ); précédent : 001D04; suivant : 001D06Preparation of monoclonal antibodies for the diagnosis of influenza A infection using different immunization protocols
Auteurs : E. Vare Ková ; T. Betáková ; V. Mucha ; L. Soláriková ; F. Kostolansk ; M. Waris [Finlande] ; G. RussSource :
- Journal of Immunological Methods [ 0022-1759 ] ; 1995.
English descriptors
- Teeft :
- Acta virol, Affinity value, Antigen detection, Antigen specificity, Assay, Clinical specimens, Clone, Competitive pattern, Competitive radioimmunoassay, Constant amount, Cyclophosphamide, Cyclophosphamide treatment, Detection system, Detector, Detector antibody, Different immunization protocols, Different subtypes, Epitope, Fetal calf serum, Fluoroimmunoassay, Fourth approach, High affinity, High proportion, Highest proportion, Hlnl, Hybridoma, Iibl, Iih3, Immune, Immune response, Immune responses, Immunization, Immunization protocol, Immunization protocols, Immunization schemes, Immunological, Immunological methods, Influenza, Influenza virus, Influenza viruses, Mabs, Methods enzymol, Methods section, Mice tolerized, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Myeloma cells, Positive reaction, Radioimmunoassay, Radioimmunoprecipitation assay, Rapid detection, Rapid diagnosis, Reference antigen, Reference detector antibody, Relative amount, Relative number, Rus, Sequential immunization, Solid phase, Specific clones, Spleen cells, Subtypes, Surface antigens, Viral, Viral antigen, Viral particles, Viral strains, Virus, Weak immunogens, Whole virus.
Abstract
Abstract: Four immunization protocols were used to obtain cross-reactive influenza type A-specific monoclonal antibodies: (1) repeated administration of purified influenza virus, (2) immunization with bromelain-treated viral particles free of HA and NA, (3) sequential immunization with two strains of different subtypes, and (4) immunization with bromelain-treated particles following tolerization of mice to surface glycoproteins by cyclophosphamide. The fourth approach was shown to be the most effective since a high proportion of hybridomas producing cross-reactive influenza virus type A-specific MAbs were obtained. MAbs of type A specificity were immunochemically characterized and examined for their ability to detect virus in clinical specimens. It was demonstrated that two pairs of the newly prepared MAbs provided excellent reagents for viral detection in clinical specimens using time-resolved fluoroimmunoassay.
Url:
DOI: 10.1016/0022-1759(94)00307-I
Affiliations:
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Vare Ková</name><affiliation><wicri:noCountry code="subField">Republic</wicri:noCountry></affiliation></author><author><name sortKey="Betakova, T" sort="Betakova, T" uniqKey="Betakova T" first="T." last="Betáková">T. Betáková</name><affiliation><wicri:noCountry code="subField">Republic</wicri:noCountry></affiliation></author><author><name sortKey="Mucha, V" sort="Mucha, V" uniqKey="Mucha V" first="V." last="Mucha">V. Mucha</name><affiliation><wicri:noCountry code="subField">Republic</wicri:noCountry></affiliation></author><author><name sortKey="Solarikova, L" sort="Solarikova, L" uniqKey="Solarikova L" first="L." last="Soláriková">L. Soláriková</name><affiliation><wicri:noCountry code="subField">Republic</wicri:noCountry></affiliation></author><author><name sortKey="Kostolansk, F" sort="Kostolansk, F" uniqKey="Kostolansk F" first="F." last="Kostolansk">F. Kostolansk</name><affiliation><wicri:noCountry code="subField">Republic</wicri:noCountry></affiliation></author><author><name sortKey="Waris, M" sort="Waris, M" uniqKey="Waris M" first="M." last="Waris">M. Waris</name><affiliation wicri:level="4"><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Virology, University of Turku, SF-20520 Turku</wicri:regionArea><orgName type="university">Université de Turku</orgName><placeName><settlement type="city">Turku</settlement><region type="région" nuts="2">Finlande occidentale</region></placeName></affiliation></author><author><name sortKey="Russ, G" sort="Russ, G" uniqKey="Russ G" first="G." last="Russ">G. Russ</name><affiliation><wicri:noCountry code="subField">Republic</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">180</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="107">107</biblScope><biblScope unit="page" to="116">116</biblScope></imprint><idno type="ISSN">0022-1759</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acta virol</term><term>Affinity value</term><term>Antigen detection</term><term>Antigen specificity</term><term>Assay</term><term>Clinical specimens</term><term>Clone</term><term>Competitive pattern</term><term>Competitive radioimmunoassay</term><term>Constant amount</term><term>Cyclophosphamide</term><term>Cyclophosphamide treatment</term><term>Detection system</term><term>Detector</term><term>Detector antibody</term><term>Different immunization protocols</term><term>Different subtypes</term><term>Epitope</term><term>Fetal calf serum</term><term>Fluoroimmunoassay</term><term>Fourth approach</term><term>High affinity</term><term>High proportion</term><term>Highest proportion</term><term>Hlnl</term><term>Hybridoma</term><term>Iibl</term><term>Iih3</term><term>Immune</term><term>Immune response</term><term>Immune responses</term><term>Immunization</term><term>Immunization protocol</term><term>Immunization protocols</term><term>Immunization schemes</term><term>Immunological</term><term>Immunological methods</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Mabs</term><term>Methods enzymol</term><term>Methods section</term><term>Mice tolerized</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Monoclonal antibody</term><term>Myeloma cells</term><term>Positive reaction</term><term>Radioimmunoassay</term><term>Radioimmunoprecipitation assay</term><term>Rapid detection</term><term>Rapid diagnosis</term><term>Reference antigen</term><term>Reference detector antibody</term><term>Relative amount</term><term>Relative number</term><term>Rus</term><term>Sequential immunization</term><term>Solid phase</term><term>Specific clones</term><term>Spleen cells</term><term>Subtypes</term><term>Surface antigens</term><term>Viral</term><term>Viral antigen</term><term>Viral particles</term><term>Viral strains</term><term>Virus</term><term>Weak immunogens</term><term>Whole virus</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Four immunization protocols were used to obtain cross-reactive influenza type A-specific monoclonal antibodies: (1) repeated administration of purified influenza virus, (2) immunization with bromelain-treated viral particles free of HA and NA, (3) sequential immunization with two strains of different subtypes, and (4) immunization with bromelain-treated particles following tolerization of mice to surface glycoproteins by cyclophosphamide. The fourth approach was shown to be the most effective since a high proportion of hybridomas producing cross-reactive influenza virus type A-specific MAbs were obtained. MAbs of type A specificity were immunochemically characterized and examined for their ability to detect virus in clinical specimens. It was demonstrated that two pairs of the newly prepared MAbs provided excellent reagents for viral detection in clinical specimens using time-resolved fluoroimmunoassay.</div></front></TEI><affiliations><list><country><li>Finlande</li></country><region><li>Finlande occidentale</li></region><settlement><li>Turku</li></settlement><orgName><li>Université de Turku</li></orgName></list><tree><noCountry><name sortKey="Betakova, T" sort="Betakova, T" uniqKey="Betakova T" first="T." last="Betáková">T. Betáková</name><name sortKey="Kostolansk, F" sort="Kostolansk, F" uniqKey="Kostolansk F" first="F." last="Kostolansk">F. Kostolansk</name><name sortKey="Mucha, V" sort="Mucha, V" uniqKey="Mucha V" first="V." last="Mucha">V. Mucha</name><name sortKey="Russ, G" sort="Russ, G" uniqKey="Russ G" first="G." last="Russ">G. Russ</name><name sortKey="Solarikova, L" sort="Solarikova, L" uniqKey="Solarikova L" first="L." last="Soláriková">L. Soláriková</name><name sortKey="Vare Kova, E" sort="Vare Kova, E" uniqKey="Vare Kova E" first="E." last="Vare Ková">E. Vare Ková</name></noCountry><country name="Finlande"><region name="Finlande occidentale"><name sortKey="Waris, M" sort="Waris, M" uniqKey="Waris M" first="M." last="Waris">M. Waris</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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